What is Cell Cultivation?

  1. Replacing the culture medium daily
    Sucking and removing old culture medium without peeling cells and adding new culture medium
  2. Conducting passages every few days
    Uniformly distributing proliferated cells into multiple culture vessels according to the desired size and density
1. Requires much time for training 2. Requires continuous work / Significant burden on researchers
Process Flow of the Automated Cell Culture System / 1. Cell proliferation 2. Passage (Cell division) 3. Medium change (Cell nutrition)

An automated cell culture system, which continuously supplies cells, has been developed.

What is Cell Cultivation?

  1. Replacing the culture medium daily
    Sucking and removing old culture medium without peeling cells and adding new culture medium
  2. Conducting passages every few days
    Uniformly distributing proliferated cells into multiple culture vessels according to the desired size and density
Process Flow of the Automated Cell Culture System / 1. Cell proliferation 2. Passage (Cell division) 3. Medium change (Cell nutrition)
1. Requires much time for training 2. Requires continuous work / Significant burden on researchers

An automated cell culture system, which continuously supplies cells, has been developed.

Basic functions for automating cell culturing processes

Automating manual techniques
Reproducing techniques by thoroughly analyzing manual work and utilizing our manipulation control technique

Ensuring repeatability
Enabling tests under the same conditions by stable mechanical movement

Improving observation functions
Observing cells using a microscope with image capturing function

Measures against biohazards
Class II safety cabinet-equivalent performance by HEPA-filtering of exhaust gas

Measures against contamination
Preventing contamination by periodic decontamination work and UV irradiation of the unit

Scheduling
Automatically conducting preset movements and maintaining history records of each culture vessel

Basic functions for automating cell culturing processes

Automating manual techniques
Reproducing techniques by thoroughly analyzing manual work and utilizing our manipulation control technique

Improving observation functions
Observing cells using a microscope with image capturing function

Measures against contamination
Preventing contamination by periodic decontamination work and UV irradiation of the unit

Ensuring repeatability
Enabling tests under the same conditions by stable mechanical movement

Measures against biohazards
Class II safety cabinet-equivalent performance by HEPA-filtering of exhaust gas

Scheduling
Automatically conducting preset movements and maintaining history records of each culture vessel

Advantages of this system

Compact size

Achieved a compact size that can be easily installed in a laboratory by designing structures that enable replacement with a clean bench

Fully automated passages

Realized fully automated passages, which are difficult in a conventional system, by automatically determining the timing for passages

Enabling unique experiments

Modular design
Specific programming

Developed an easy-to-use system by modularizing the system into selectable modules and adopting programmable operations in accordance with customer needs

Advantages of this system

Compact size

Achieved a compact size that can be easily installed in a laboratory by designing structures that enable replacement with a clean bench

Fully automated passages

Realized fully automated passages, which are difficult in a conventional system, by automatically determining the timing for passages

Enabling unique experiments

Modular design
Specific programming

Developed an easy-to-use system by modularizing the system into selectable modules and adopting programmable operations in accordance with customer needs

Specification

Top view. Body depth: 1116 mm, width 2690 mm 600 mm maintenance space is required around the machine Control panel has 100V supply port, 200V supply port Body height: 2350 mm
Front view. Height 2350mm. 300 mm maintenance space required at the top There is a CO2 supply port

Cultivating process

Culture medium replacement, passage, cell observation

Target cell

Adhesive cell: ES/iPS cell (colony/single cell), etc.

Heat insulation

Max. 12 types
(Three 500 ml bottles or Twelve 50 ml tubes)

Culture vessel

φ100 mm dish , six-well plate

Storage capacity

Max. 60 dishes (Ten φ100 mm dishes/rack, 6 racks)
Max. 27 well plates (9 pieces/rack, 3 racks)
* Depending on the combination

Dedicated supplies

Pipette (rack supply type): 75 units

Cultivation plan

Cultivating process can be set using the schedule function.

Biohazard

Cabinet class II equivalent (Clean 100 equivalent in the unit)
Partly evaluated according to our own standards

Decontamination

UV irradiation, peracetic acid fumigation

Cell observation

Image storage, passage timing determination, cell count

Main unit dimensions

W 1,998 x D 1,116 x H 2,350 mm
(Main unit only, excluding main control panel)

Main unit weight

Total: approx. 1,150 kg
(Including main control panel, excluding backup power supply and control panel)
Main unit: approx. 980 kg

Utilities

  • Three-phase 200 VAC, 30 A: One system
  • 100 VAC, 20 A: One system
  • 100 VAC, 15 A: Two systems
    (Two 2-pin socket with a ground)
  • φ8 (I.D.) hose for CO2 gas supply
    (The main unit has a φ8 mm hose barb fitting.)