What is Cell Cultivation?

  1. Changing culture medium daily
    Suctioning and removing old culture medium, without peeling off cells, and adding new culture medium
  2. Conducting cell passages every few days
    Uniformly disseminating proliferated cells into multiple culture vessels according to desired size and density
1. Requires much time for training 2. Requires continuous work / Significant burden on researchers
Process Flow of Automated Cell Culture System / 1. Cell proliferation 2. Cell passage (Cell division) 3. Medium change (Cell nutrition)

Automated cell culture system that continuously supplies cells

What is Cell Cultivation?

  1. Changing culture medium daily
    Suctioning and removing old culture medium, without peeling off cells, and adding new culture medium
  2. Conducting cell passages every few days
    Uniformly disseminating proliferated cells into multiple culture vessels according to desired size and density
Process Flow of Automated Cell Culture System / 1. Cell proliferation 2. Cell passage (Cell division) 3. Medium change (Cell nutrition)
1. Requires much time for training 2. Requires continuous work / Significant burden on researchers

Automated cell culture system that continuously supplies cells

Basic Functions for Automating Cell Culturing Processes

Clean cabinet
HEPA filter keeps clean and safe environment equivalent to class II safety cabinet

Refrigerator & Warmer
Refrigerators can store Max. 4 500-mL bottles at 4°C. Warmers are provided to warm liquid temporarily.

Incubator
Max. 60 100-mm dishes (or max. 27 6-well plates) can be stored in CO2 incubator

Cell observation
Deep-learning AI technology leveraged to find cells’ conditions or count cell number

Dispensing & Swinging
Special 10mL pipette is suitable for separating cells. Swing equipment to blend suspension in a dish.

Centrifuging
A centrifuge is implemented for automated cell passage. Considering its effect on cells, it's placed far from the incubator.

Basic Functions for Automating Cell Culturing Processes

Clean cabinet
HEPA filter keeps clean and safe environment equivalent to class II safety cabinet

Incubator
Max. 60 100-mm dishes (or max. 27 6-well plates) can be stored in CO2 incubator

Dispensing & Swinging
Special 10mL pipette is suitable for separating cells. Swing equipment to blend suspension in a dish.

Refrigerator & Warmer
Refrigerators can store Max. 4 500-mL bottles at 4°C. Warmers are provided to warm liquid temporarily.

Cell observation
Deep-learning AI technology leveraged to find cells’ conditions or count cell number

Centrifuging
A centrifuge is implemented for automated cell passage. Considering its effect on cells, it's placed far from the incubator.

Advantages of New System

Automating manual techniques

Reproducing techniques by thoroughly analyzing manual work and utilizing proposed manipulation control technique

Compact size

Achieved a compact size that easily fits into laboratories by designing a structure that is interchangeable with a standard clean bench

Scheduling

Automatically conducting preset movements and maintaining history records of each culture vessel

Advantages of New System

Automating manual techniques

Reproducing techniques by thoroughly analyzing manual work and utilizing proposed manipulation control technique

Compact size

Achieved a compact size that easily fits into laboratories by designing a structure that is interchangeable with a standard clean bench

Scheduling

Specific programming

Automatically conducting preset movements and maintaining history records of each culture vessel

Specification

W2,000 x D1,130 x H2,412mm(main apparatus only, excluding PC rack)

Cultivating process

Culture medium change, cell passage, cell observation

Target cell

Adhesive cell: ES/iPS cell (colony/single cell), etc.

Heat insulation

4-500ml bottles

Culture vessel

Max. 60 Φ100-mm dishes (or max. 27 6-well plates) can be stored

Dedicated supplies

10mL pipettes (Max. 100 bottles)

Cultivation plan

Cultivating process can be set using scheduling function

Biohazard

Cabinet class II equivalent (Clean 100 equivalent within the unit)
Partly evaluated according to our own standards

Decontamination

UV irradiation, peracetic acid fumigation

Cell observation

Image storage, passage timing determination, cell count

Main unit dimensions

W 2,000 x D 1,130 x H 2,412 mm
(main apparatus only, excluding PC rack)

Utilities

  • Three-phase 200 VAC, 30 A: One system
  • 100 VAC, 20 A: One system
  • 100 VAC, 15 A: Two systems
    (two 2-pin sockets with a ground)
  • CO2 gas supply : One system
    (outer diameter φ12-mm hose barb fitting)