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A image of bacteria & viruses

Effect

World’s First*¹ Clarification of Part of the
Inactivation Mechanism of SARS-CoV-2 by nanoe™ (Hydroxyl Radicals Contained in Water) Technology

Through collaborative research with Associate Professor Mayo Yasugi of the Graduate School of Veterinary Science, Osaka Metropolitan University, it has revealed for the first time that the structural collapse of the SARS-CoV-2 is one of the causes of the inactivation of the viruses through exposure to nanoe™.

[PRESS RELEASE] World’s First Clarification of Part of the Inactivation Mechanism of SARS-CoV-2 by nanoe™

nanoe™ destroys the viruses in pieces

nanoe™ comes in contact with the surface of viruses, damaging proteins on the cell surface including spike protein that get viruses bound to cells, as well as damaging the envelope.

An image of nanoe™ contacting the surface of the virus
AN image of nanoe™ damaging spike proteins on the cell surface

Progressive degradation of proteins on the virus surface and damage to the envelop expand deformation. The envelope collapses.

An image of progressive degradation of proteins on the virus surface
An image of the envelope collapsing

Degradation of internal proteins including nucleocapsid proteins and viral genomic RNA. The virus is destroyed in pieces.

An image of degradation of internal proteins including nucleocapsid proteins
An image of degradation of internal proteins including viral genomic RNA

Which process does nanoe™ inhibit infection mechanism of SARS CoV-2?

The mechanism of SARS-CoV-2 infecting the cell

An image of virus and host cell approaching

1. Virus approaches host cell

An image of viruses binding to the host cell receptor

2. Virus binds to the host cell receptor

An image of virus invading in the host cell and replicates

3. Virus invades in the host cell and replicates

What happens when nanoe™ exists?

An image of virus destroyed in pieces by nanoe™ effect

1. Virus destroyed in pieces by nanoe™ effect

An image of virus destroyed in pieces cannot bind with cell

2. Virus destroyed in pieces cannot bind with cell

An image of no binding, leading the virus not being able to invade into the cell and there is no infection

3. As no binding, the virus cannot invade into the cell and there is no infection

By nanoe™, certain viruses became less infectious

nanoe™ does not target specific molecules or structures of viruses, but damages SARS-CoV-2 by acting at multiple steps on the envelope, proteins, and genomic RNA that make up viruses. Viruses damaged by exposure to nanoe™ lose their ability to bind to host cell receptors, thereby becoming less infectious. These series of phenomena are considered to be part of the mechanism of how the SARS-CoV-2 is inactivated by nanoe™.

Paper about inactivation mechanism of SARS-CoV-2 by nanoe™

Inhibits activity of airborne,
adhered bacteria1-3 & viruses4-6

Certain bacteria and viruses are far too small to see

The difference in size between a certain bacteria and viruses is about the same as the difference between apples and sesame seeds.

A figure of size comparision between viruses and bacteira

The virus survives different periods on different surfaces

Survival period varies depending on surface, from 3 hours to 7 days.

Type of substances

Survival period

Paper, tissue

3 hours

Copper surface*

4 hours

Cardboard surface

24 hours

Cloth surface

2 days

Plastic surface

3 days

Glass surface

4 days

Banknote surface

4 days

Outside of surgical mask

7 days

*Copper naturally degrades certain bacteria and viruses.
Survival period differs depending on surface irregularities.
Viruses survive longer on smooth surfaces than on irregular surfaces.
Source: https://www.businessinsider.com/coronavirus-lifespan-on-surfaces-graphic-2020-3

Effects on certain bacteria & viruses

Airborne bacteria
Staphylococcus aureus1

A graph showing that nanoe™ X is highly effective against the airborne bacteria Staphylococcus aureus

Airborne viruses
bacteriophageΦχ1744

A graph showing that nanoe™ X is highly effective against the airborne bacteria bacteriophage Φχ174

Adhered bacteria
O1572

A graph showing that nanoe™ X is highly effective against the adhered bacteria O157

Adhered viruses
Influenza virus H1N1 subtype5

A graph showing that nanoe™ X is highly effective against adhered influenza virus H1N1 subtype

MRSA3

A graph showing that nanoe™ X is highly effective against the adhered bacteria MRSA

Poliovirus type1 (Lsc-2ab)6

A graph showing that nanoe™ X is highly effective against the adhered virus Poliovirus type 1 (Lsc-2ab)

1Airborne bacteria (Staphylococcus aureus). Testing organisation: Kitasato Research Center for Environmental Science. Testing method: The number of bacteria was measured after direct exposure in an approximately 25 m3-sized airtight test chamber. Inhibition method: nanoe™ released. Target substance: Airborne bacteria. Test result: Inhibited by at least 99.7% in 4 hours. (24_0301_1)
2Adhered bacteria (O157). Testing organisation: Japan Food Research Laboratories. Testing method: Measured the number of bacteria adhered to a cloth in an approximately 45 L-sized airtight test chamber. Inhibition method: nanoe™ released. Target substance: Adhered bacteria. Test result: Inhibited by at least 99.99% in 1 hour. (208120880_001)
3Adhered bacteria (MRSA). Testing organisation: Japan Food Research Laboratories. Testing method: Measured the number of bacteria adhered to a cloth in an approximately 45-L-sized airtight test chamber. Inhibition method: nanoe™ released. Target substance: Adhered bacteria. Test result: Inhibited by at least 99.99% in 1 hour. (208120880_002)
4Airborne viruses (bacteriophageΦχ174). Testing organisation: Kitasato Research Center for Environmental Science. Testing method: The number of viruses was measured after direct exposure in an approximately 25 m3-sized airtight test chamber. Inhibition method: nanoe™ released. Target substance: Airborne viruses. Test result: Inhibited by at least 99.7% in 6 hours. (24_0300_1)
5Adhered virus (Influenza virus H1N1 subtype). Testing organisation: Kitasato Research Center for Environmental Science. Testing method: Measured the number of viruses adhered to a cloth in an approximately 1 m3-sized airtight test chamber. Inhibition method: nanoe™ released. Target substance: Adhered viruses. Test result: Inhibited by at least 99.9% in 2 hours. (21_0084_1)
6Adhered viruses (Poliovirus type1(Lsc-2ab)). Testing organisation: Kitasato Research Center for Environmental Science. Testing method: Measured the number of viruses adhered to a cloth in an approximately 45 L-sized airtight test chamber. Inhibition method: nanoe™ released. Target substance: Adhered viruses. Test result: Inhibited by at least 99.7% in 2 hours. (22_0096)

Results may vary based on usage and seasonal and environmental variables (temperature and humidity). nanoe™ X and nanoe™ inhibit activity or growth of pollutants, but do not prevent illness.

Evidence

Viruses

nanoe™

Target

Results*¹

Test space

Exposure
time (hr)

Testing organisation

Report No.

PDF

Adhering

Novel coronavirus
 (SARS-CoV-2)

99.7%
inhibited

45 L

3

Osaka Prefecture University

Bird flu virus
(H5N1 and H9N2 subtypes)

99.9%
inhibited

45 L

4

Obihiro University of Agriculture
and Veterinary Medicine,
Research Center for Animal Hygiene and Food Safety

Swine-origin influenza virus
(A/Narita/2009 (H1N1) pdm)

99%
inhibited

45 L

6

Influenza virus
(H1N1 subtype)

99.9%
inhibited

45 L

4

Japan Food Research Laboratories

208040534-001

99.9%
inhibited

1,000 L

2

Kitasato Research Center
for Environmental Science

21_0084_1

Bacteriophage
Φχ174

99.9%
inhibited

10,000 L

24

Kitasato Research Center
for Environmental Science

21_0143_4

99.8%
inhibited

Approx.
25 m³

8

Japan Food Research Laboratories

13001265005-01

Coxackievirus
(B6)

99.1%
inhibited

45 L

2

Kitasato Research Center
for Environmental Science

22_0085

Poliovirus type 1
(Lsc-2ab)

99.7%
inhibited

45 L

2

22_0096

Encephalomyocarditis virus

99.9%
inhibited

45 L

6

Charles River Biopharmaceutical
Services GmbH

Virus clearance test

Porcineparvovirus

99.7%
inhibited

45 L

6

Xenotropic murine leukemia virus

99.999%
inhibited

45 L

6

Pseudororabies virus

99.9%
inhibited

45 L

6

Canine distemper virus

99.7%
inhibited

45 L

4

Rakuno Gakuen University,
School of Veterinary Medicine

Canine parvovirus

99.8%
inhibited

45 L

6

Canine herpesvirus

99.5%
inhibited

45 L

4

Canine adenovirus

99.4%
inhibited

45 L

4

Yamaguchi University,
Faculty of Agriculture

Feline coronavirus

99.3%
inhibited

45 L

2

Feline calicivirus
(related to norovirus)

99.9%
inhibited

25 L

2

Japan Food Research Laboratories

207031493-001

Airborne

Bacteriophage
Φχ174

99%
inhibited

1,000 L

1.5

Kitasato Research Center
for Environmental Science

20_0154_1

99.2%
inhibited

10,000 L

4

21_0147

99.74%
inhibited

Approx.
25 m³

6

24_0300_1

nanoe™ X

Target

Results*¹

Test space

Exposure
time (hr)

Testing organisation

Report No.

PDF

Adhering

Novel coronavirus
(SARS-CoV-2)

99.7%
inhibited

45 L

0.2

Osaka Prefecture University

99.9%
inhibited

45 L

0.2

Texcell

1140-01 A1

91.4%
inhibited

6.7 m³

0.5

1140-01 C3

Bird flu virus
(H5N1 and H9N2 subtypes)

99.9%
inhibited

45 L

2

Obihiro University of Agriculture
and Veterinary Medicine,
Research Center for Animal Hygiene and Food Safety

Swine-origin influenza virus
(A/Narita/2009 (H1N1) pdm)

99%
inhibited

45 L

2

Influenza virus
(H1N1 subtype)

99.9%
inhibited

1,000 L

2

Kitasato Research Center
for Environmental Science

21_0084_1

Bacteriophage
Φχ174

99.9%
inhibited

10,000 L

2

Kitasato Research Center
for Environmental Science

21_0143_4

99.8%
inhibited

Approx.
25 m³

2

Japan Food Research Laboratories

13001265005-01

Coxackievirus
(B6)

99.1%
inhibited

45 L

2

Kitasato Research Center
for Environmental Science

22_0085

Poliovirus type 1
(Lsc-2ab)

99.7%
inhibited

45 L

2

22_0096

Encephalomyocarditis virus

99.9%
inhibited

45 L

2

Charles River Biopharmaceutical
Services GmbH

Virus clearance test

Porcineparvovirus

99.7%
inhibited

45 L

1

Xenotropic murine leukemia virus

99.999%
inhibited

45 L

1

Pseudororabies virus

99.9%
inhibited

45 L

6

Canine distemper virus

99.7%
inhibited

45 L

4

Rakuno Gakuen University,
School of Veterinary Medicine

Canine parvovirus

99.8%
inhibited

45 L

6

Canine herpesvirus

99.5%
inhibited

45 L

4

Canine adenovirus

99.4%
inhibited

45 L

4

Yamaguchi University,
Faculty of Agriculture

Feline coronavirus

99.3%
inhibited

45 L

2

Feline calicivirus
(related to norovirus)

99.9%
inhibited

25 L

2

Japan Food Research Laboratories

207031493-001

Enterovirus 71
(EV71)
(hand-foot-and-mouth disease)

99.1%
inhibited

61 L

4

Wuhan Institute of Virology, CAS

MVCCC-2019/T4

99.9%
inhibited

61 L

6

Coxsackievirus A16
(CA16)
(hand-foot-and-mouth disease)

99.3%
inhibited

61 L

2

Wuhan Institute of Virology, CAS

MVCCC-2019/T4

99.9%
inhibited

61 L

4

Human coronavirus
(HCoV-229E)

99.2%
inhibited

68 L

4

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00465

Airborne

Bacteriophage
Φχ174

99%
inhibited

1,000 L

1.5

Kitasato Research Center
for Environmental Science

20_0154_1

99.2%
inhibited

10,000 L

4

21_0147

99.2%
inhibited

Approx.
25 m³

6

24_0300_1

nanoe™ X (nanoe™ X Generator Mark 2)

Target

Results*¹

Test space

Exposure
time (hr)

Testing organisation

Report No.

PDF

Adhering

Enterovirus (EV71)

99.9%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00439

Enterovirus (EV71)

99.9%
inhibited

30 m³

6

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00439

Enterovirus (EV71)

97.6%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J1911WT8888-04860-01

Enterovirus (EV71)

99.9%
inhibited

30 m³

6

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00453

Coxsackie virus (CA16)

99.9%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00439

Coxsackie virus (CA16)

99.9%
inhibited

30 m³

6

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00439

Coxsackie virus (CA16)

95.4%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J1911WT8888-004860-02

Coxsackie virus (CA16)

99.9%
inhibited

30 m³

6

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00453

Coronavirus (HCoV-229E)

99.9%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00544-01

Coronavirus (HCoV-229E)

99.9%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00889

Herpes simplex virus

99.9%
inhibited

30 m³

4

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00889

Airborne

Enterovirus (EV71)

95.4%
inhibited

30 m³

1.5

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00439

Enterovirus (EV71)

96.9%
inhibited

30 m³

1.5

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00889

Influenza virus (H1N1)

95.4%
inhibited

30 m³

1.5

China Electronic Product Reliability and Environmental Testing Research Institute

J2002WT8888-00439

Influenza virus (H1N1)

98.3%
inhibited

30 m³

1.5

China Electronic Product Reliability and Environmental Testing Research Institute

J2003WT8888-00889

nanoe™ X (nanoe™ X Generator Mark 3)

Target

Results*¹

Test space

Exposure
time (hr)

Testing organisation

Report No.

PDF

Adhering

Bacteriophage

Inhibited
98.81%

Approx.
139.3m³

4

SGS Inc

SHES210901902584

MS2 Phage Virus

Inhibited
99.99%

Approx.
25m³

2

Shokukanken, Inc.

227131N

Bacteria

nanoe™

Target

Results*¹

Test space

Exposure
time (hr)

Testing organisation

Report No.

PDF

Adhering

Enterohemorrhagic Escherichia coli
(O157)

99.99%
inhibited

45 L

1

Japan Food Research Laboratories

208120880-001

99.99%
inhibited

45 L

2

Eurofins

2010/456-3

Enterohemorrhagic
Escherichia coli
(O157)
Note: Refrigerator

99.99%
inhibited

45 L

2

2010/456-4

Methicillin-resistant Staphylococcus aureus
(MRSA)

99.99%
inhibited

45 L

1

Japan Food Research Laboratories

208120880-002

99.99%
inhibited

45 L

1

Eurofins

2010/456-1

99.99%
inhibited

45 L

2

Osaka Prefecture University

Escherichia coli

99.99%
inhibited

45 L

2

Panasonic Product Analysis Center

E02-080303IN-01

Staphylococcus aureus

99.99%
inhibited

1,000 L

2

Kitasato Research Center
for Environmental Science

20_0154_2

99.2%
inhibited

10,000 L

2

Panasonic Product Analysis Center

E02-090701IN-01

99%
inhibited

10,000 L

2

Japan Food Research Laboratories

209071031-001

99.1%
inhibited

Approx.
23 m³

2

13044083003-01

99%
inhibited

Approx.
23 m³

1

SGS

SHES160600431171

Multiple-drug-resistant Pseudomonas aeruginosa
 (MDRP)

99%
inhibited

45 L

1

Toho University Faculty of Medicine,
Department of Infection Control
and Prevention

Multi-drug resistant Acinetobacter baumannii
(MDRAB)

99%
inhibited

45 L

2

Bacillus

99.7%
inhibited

45 L

1

Japan Food Research Laboratories

11000924001-01

Micrococcus

99.9%
inhibited

45 L

0.5

11000924001-02

Serratia

99.9%
inhibited

45 L

0.5

11000924001-03

Kocuria

99.9%
inhibited

45 L

0.5

11000922001-01

methicillin-resistant staphylococcus pseudintermedius
(MRSP)

99.5%
inhibited

45 L

0.5

Nippon Medical School,
Nippon Veterinary and Life Science University

Bordetella bronchiseptica

99.9%
inhibited

45 L

0.5

Pasteurella multocida

99.9%
inhibited

45 L

1

Listeria monocytogenes

99.9%
inhibited

45 L

2

Osaka Prefecture University

Bacillus subtilis

99.9%
inhibited

45 L

4

Mycobacterium smegmatis

99.9%
inhibited

45 L

4

Nocardia asteroids

99.9%
inhibited

45 L

4

Neisseria gonorrhoeae

99.9%
inhibited

45 L

1

Salmonella enterica
subsp. enterica

99.9%
inhibited

45 L

2

Haemophilus influenza

99.9%
inhibited

45 L

1

Campylobacter jejuni

99.9%
inhibited

45 L

4

Airborne

Staphylococcus aureus

99.9%
inhibited

1,000 L

0.34

Kitasato Research Center
for Environmental Science

20_0154_1

99%
inhibited

10,000 L

4

21_0142

99.9%
inhibited

10,000 L

3

21_0044

99.7%
inhibited

Approx.
25 m³

4

24_0301_1

nanoe™ X

Target

Results*¹

Test space

Exposure
time (hr)

Testing organisation

Report No.

PDF

Adhering

Enterohemorrhagic Escherichia coli
(O157)

99.99%
inhibited

45 L

1

Japan Food Research Laboratories

208120880-001

99.9%
inhibited

45 L

2

Eurofins

2010/456-3

Enterohemorrhagic
Escherichia coli
(O157)
Note: Refrigerator

99.99%
inhibited

45 L

2

2010/456-4

Methicillin-resistant Staphylococcus aureus
(MRSA)

99.99%
inhibited

45 L

1

Japan Food Research Laboratories

208120880-002

99.99%
inhibited

45 L

1

Eurofins

2010/456-1

99.9%
inhibited

45 L

2

Osaka Prefecture University

Escherichia coli

99.9%
inhibited

45 L

1

Panasonic Product Analysis Center

E02-080303IN-01

Staphylococcus aureus

99.99%
inhibited

1,000 L

0.34

Kitasato Research Center
for Environmental Science

20_0154_2

99.2%
inhibited

10,000 L

24

Panasonic Product Analysis Center

E02-090701IN-01

99%
inhibited

10,000 L

24

Japan Food Research Laboratories

209071031-001

99.1%
inhibited

Approx.
23 m³

8

13044083003-01

99%
inhibited

Approx.
23 m³

8

SGS

SHES160600431171

99.91%
inhibited

24 m³

8

Panasonic Product Analysis Center

1V332-170703-F04

Multiple-drug-resistant Pseudomonas aeruginosa
 (MDRP)

99%
inhibited

45 L

2

Toho University Faculty of Medicine,
Department of Infection Control
and Prevention

Multi-drug resistant Acinetobacter baumannii
(MDRAB)

99%
inhibited

45 L

2

Bacillus

99.7%
inhibited

45 L

0.5

Japan Food Research Laboratories

11000924001-01

Micrococcus

99.9%
inhibited

45 L

2

11000924001-02

Serratia

99.9%
inhibited

45 L

2

11000924001-03

Kocuria

99.9%
inhibited

45 L

1

11000922001-01

methicillin-resistant staphylococcus pseudintermedius
(MRSP)

99.5%
inhibited

45 L

2

Nippon Medical School,
Nippon Veterinary and Life Science University

Bordetella bronchiseptica

99.9%
inhibited

45 L

2

Pasteurella multocida

99.9%
inhibited

45 L

1

Listeria monocytogenes

99.9%
inhibited

45 L

2

Osaka Prefecture University

Bacillus subtilis

99.9%
inhibited

45 L

4

Mycobacterium smegmatis

99.9%
inhibited

45 L

4

Nocardia asteroids

99.9%
inhibited

45 L

4

Neisseria gonorrhoeae

99.9%
inhibited

45 L

1

Salmonella enterica subsp. enterica

99.9%
inhibited

45 L

2

Haemophilus influenza

99.9%
inhibited

45 L

1

Campylobacter jejuni

99.9%
inhibited

45 L

4

Airborne

Staphylococcus aureus

99.9%
inhibited

1,000 L

0.34

Kitasato Research Center
for Environmental Science

20_0154_1

99%
inhibited

10,000 L

4

21_0142

99.9%
inhibited

10,000 L

3

21_0044

99.7%
inhibited

Approx.
25 m³

4

24_0301_1

nanoe™ X: improving protection 24/7

Effects of nanoe™ X—unique Panasonic technology